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Next Generation Sequencing Request Form

For pricing and experimental design, contact Daniel Johnson

Ìý	314 Chip	316 Chip	318 chip	P1 Chip
Coverage	300K-550K reads	1-3 Million reads	2-5 Million reads	60-90ÌýMillion reads
Data File Size	30-100 MB	300 Mb-1 GB	500 Mb-2 GB	~15 GB
# samples	# samples can be barcoded depends on coverage required
RNA Seq	Ìý			2 µg of DNase treatedÌýRNA, for low input samplesÌýcontact us
chIPseq	1.5 ng-30 ng of chIP DNA, for low input samples contact us
miRNAseq	2 µg of enriched miRNA or 3Ìýµg of total RNA Platform used will depend on the number of samples submitted.
Targeted Seq	200 ng-1 µg genomic DNA, platform used will depend on the number of samples.
Exomes	Ìý			2 µg of genomic DNA
Whole Genome Sequencing	Ìý			500 ng-2 µg genomic DNA, for low input contact us
Special Instructions	Prior arrangements must be made for data analysis; RNA samples must be DNase treated before submitting; Samples must be quantified by fluorescence. All RNA samples must have a RIN of 8 or better from Agilent Bioanalyzer, for low input samples contact us.